Probing nascent protein folding
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چکیده
Deciphering the mechanisms by which proteins fold remains a substantial challenge for structural biology. In particular, very little is known about cotranslational folding, a process in which folding begins as the nascent protein is still emerging from the ribosome. Recognizing the dearth of methods to study this early folding event, Clark and colleagues present a strategy to stall protein translation in vitro or in vivo using a short polypeptide ‘stall sequence’ that can be inserted at virtually any point in the sequence of a recombinant protein. The conformational state of the ribosome-bound nascent protein chain can then be probed using a variety of biophysical techniques. Article p757
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